| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells
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| Autores: |
Schwindt,Telma T.
Motta,Fabiana L.
Gabriela F.,Barnabé
Cristina G.,Massant
Guimarães,Alessander O.
Calcagnotto,Maria Elisa
Pesquero,João B.
Mello,Luiz E.
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| Data: |
2009-09-01
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| Ano: |
2009
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| Palavras-chave: |
Neural precursor cells
Neurosphere
Fibroblast growth factor 2
Epidermal growth factor
Differentiation
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| Resumo: |
Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300009
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/S0001-37652009000300009
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.81 n.3 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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